ChemDiv is proud to offer unparalleled capabilities in Drug Discovery. Our screening approach to high throughput hit hunting tremendously benefits from the application of our proprietary focused discovery sub-libraries assembled from the ChemDiv’s ever-growing collection of 1.5 million small moleculesbeing synthesized based on unique ideas of novel templates. Design of these subsets provides a very robust way for identifying and confirming hit compounds with immediate mini-SAR analyses of the hit series, which serve as a starting point for medicinal chemistry hit-to-lead exploration and lead optimization.

We have assembled a robust screening infrastructure to be able to work with targets of major therapeutics areas. Our technical park of Discovery Division includes:

Equipment

• Liquid Handling
  • Biomek 2000,
  • Biomek NX,
  • Biomek FX work station
  • 96/384-well plate readers;
    • FLIPR-Tetra (Molecular Devices)
    • Microbeta PLUS 1450 (PerkinElmer),
    • scintillation counters;
    • Victor2V and Victor 3 multimode;
    • SpectraMax Plus 96/384;
    • Mach III (Tomtec) 96-well cell harvester;
    • Beckman and GUAVA 96/384 well format Flow Cytometers.

Readout capabilities

• Luminescence: Reporter genes; ELISAs;
• Fluorescence: FLINT, FRET, TR-FRET, FP, GFP; ELISAs
• Radio-Isotope: Receptor assays (ligand-binding/competition), SPA, metabolic enzymes

Absorbance: OD, ABS spectra

Ready to deploy assays

• In vitro assays:
  • FLIPR (cytoplasmic and mitochondrial calcium),
  • LANCE (cytoplasmic cAMP/cGMP),
  • ELISA,
  • Western,
  • Kinase activity, Enzymatic activity
• Cell Assays:
  • cell growth,
  • cell cycle analysis,
  • apoptosis/necrosis,
  • in vitro chemoinvasion,
  • high content high throughput microscopy
• Molecular and Cell Biology:
  • Custom Expression / Reporter Constructs;
  • Transient/stable transfection.
  • Cell lines and primary cultures;

Custom Assay Development (per Clients’ specification)